Review



addgene plasmid  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Addgene inc addgene plasmid
    Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+hygro/pLKO%2E1+hygro+(Plasmid+%2324150)/pm41915470-258-17-17
    Average 94 stars, based on 142 article reviews
    addgene plasmid - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Fibrillarin-dependent 2'-O-methylation modulates RPS28 ribosome incorporation and oncogenic translation.
    Article Snippet: .. The shRNAs were cloned into pLKO.1-Puro and pLKO.1-Hygro (Addgene #8453 and #24150) following Addgene recommendations, with a few modifications. .. Briefly, the plasmids were digested using AgeI and EcoRI restriction enzymes (Anza, Invitrogen). shRNA oligos were annealed and ligated into the pLKO.1 backbone vector.

    Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function
    Article Snippet: .. All shRNAs were cloned into pLKO.1 hygro (a gift from Bob Weinberg; Addgene plasmid #24150, RRID:Addgene_24150). shRNA knockdown and GLuc-T2A-EGFP-expressing lines were generated through lentiviral transduction. .. Lentiviral particles were produced by co-transfecting 293T cells with pLKO.1 hygro lentiviral constructs or pLV GLuc-T2A-EGFP (VectorBuilder), along with pMDLg/pRRE, pRSV-Rev, and pMD2.g (gifts from Didier Trono.

    Article Title: Regulation of mitophagy by Fis1 and Fascin1-organized actin.
    Article Snippet: Article

    Article Title: Fibrillarin shapes oncogenic protein pools and ribosomal composition in triple-negative breast cancer
    Article Snippet: .. The shRNAs were cloned into pLKO.1-Puro and pLKO.1-Hygro (Addgene #8453 and #24150) following Addgene recommendations, with a few modifications. .. Briefly, the plasmids were digested using AgeI and EcoRI restriction enzymes (Anza, Invitrogen). shRNA oligos were annealed and ligated into the pLKO.1 backbone vector.

    Article Title: Enhanced therapeutic efficacy of anti-PD-1 blockade by targeting LAMP2A inhibits lysosomal degradation of STING and TBK1
    Article Snippet: The chemicals used were listed as follows: NH4Cl (Ammonium chloride, A9434, Sigma, St. Louis, MO, USA), Leupeptin (103476-89-7, MP Biomedicals, California, USA), MG132 (S2619, Selleck), and CHX (cycloheximide, S7418, Selleck), AKTi-1/2(AKT inhibitor VIII, HY-10355, MCE), AR7 (HY-101106, MCE), Rapamycin(553210, Sigma), D-Luciferin Sodium Salt (40901ES, Yeasen, China), C-176(SML2559, Sigma), GSK8612(SML2721, Sigma), Diclofenac Sodium (D6899, Sigma). .. Single guide RNAs (sgRNAs) were cloned into lentiCRISPR v2 (Addgene # 52961) and shRNA were cloned into pLKO.1 puro (Addgene #8453) or pLKO.1 hygro(Addgene #24150). siRNAs targeting siLAMP2A, siATG5, siATG7 and negative control (NC) siRNA transfected into cells with a riboFECTTM CP Transfection Kit (RiboBio). ..

    Plasmid Preparation:

    Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function
    Article Snippet: .. All shRNAs were cloned into pLKO.1 hygro (a gift from Bob Weinberg; Addgene plasmid #24150, RRID:Addgene_24150). shRNA knockdown and GLuc-T2A-EGFP-expressing lines were generated through lentiviral transduction. .. Lentiviral particles were produced by co-transfecting 293T cells with pLKO.1 hygro lentiviral constructs or pLV GLuc-T2A-EGFP (VectorBuilder), along with pMDLg/pRRE, pRSV-Rev, and pMD2.g (gifts from Didier Trono.

    Article Title: Compositions and methods for treating, preventing or reversing age associated inflammation and disorders
    Article Snippet: .. Four selectable markers were used to allow multiple drug selections: pLKO.1 puro (2 μg/ml) and pLKO.1 hygro (200 μg/ml) (Addgene plasmid #8453, 24150), pLKO.1 blast (5 μg/ml) (Addgene plasmid #26655), pLKO.1 neo (250 μg/ml) (Addgene plasmid 13425). pLKO-RB1-shRNA63 and pLKO-RB1-shRNA19 (Addgene plasmids #25641 and 25640).86 For FOXA1 shRNAs TRCN0000014881 (a) and TRCN0000014882 (b) were used. ..


    shRNA:

    Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function
    Article Snippet: .. All shRNAs were cloned into pLKO.1 hygro (a gift from Bob Weinberg; Addgene plasmid #24150, RRID:Addgene_24150). shRNA knockdown and GLuc-T2A-EGFP-expressing lines were generated through lentiviral transduction. .. Lentiviral particles were produced by co-transfecting 293T cells with pLKO.1 hygro lentiviral constructs or pLV GLuc-T2A-EGFP (VectorBuilder), along with pMDLg/pRRE, pRSV-Rev, and pMD2.g (gifts from Didier Trono.

    Article Title: Regulation of mitophagy by Fis1 and Fascin1-organized actin.
    Article Snippet: Article

    Article Title: Enhanced therapeutic efficacy of anti-PD-1 blockade by targeting LAMP2A inhibits lysosomal degradation of STING and TBK1
    Article Snippet: The chemicals used were listed as follows: NH4Cl (Ammonium chloride, A9434, Sigma, St. Louis, MO, USA), Leupeptin (103476-89-7, MP Biomedicals, California, USA), MG132 (S2619, Selleck), and CHX (cycloheximide, S7418, Selleck), AKTi-1/2(AKT inhibitor VIII, HY-10355, MCE), AR7 (HY-101106, MCE), Rapamycin(553210, Sigma), D-Luciferin Sodium Salt (40901ES, Yeasen, China), C-176(SML2559, Sigma), GSK8612(SML2721, Sigma), Diclofenac Sodium (D6899, Sigma). .. Single guide RNAs (sgRNAs) were cloned into lentiCRISPR v2 (Addgene # 52961) and shRNA were cloned into pLKO.1 puro (Addgene #8453) or pLKO.1 hygro(Addgene #24150). siRNAs targeting siLAMP2A, siATG5, siATG7 and negative control (NC) siRNA transfected into cells with a riboFECTTM CP Transfection Kit (RiboBio). ..

    Article Title: Interaction and Reciprocal Stabilization of H3.3 and PARP1 Promotes DNA Damage Repair and Tumor Progression in Small Cell Lung Cancer
    Article Snippet: 28 Multiple lines of evidence have highlighted the transcriptional regulatory activity of H3.3 at promoters and 29 telomeres.. However, H3.3 may also play transcriptional regulation-independent roles.. In this study, we 30 demonstrated that overexpression of H3F3A, the gene encoding H3.3, supported the pathogenesis of 31 small cell lung cancer (SCLC) by protecting PARP1 from degradation.

    Knockdown:

    Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function
    Article Snippet: .. All shRNAs were cloned into pLKO.1 hygro (a gift from Bob Weinberg; Addgene plasmid #24150, RRID:Addgene_24150). shRNA knockdown and GLuc-T2A-EGFP-expressing lines were generated through lentiviral transduction. .. Lentiviral particles were produced by co-transfecting 293T cells with pLKO.1 hygro lentiviral constructs or pLV GLuc-T2A-EGFP (VectorBuilder), along with pMDLg/pRRE, pRSV-Rev, and pMD2.g (gifts from Didier Trono.

    Generated:

    Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function
    Article Snippet: .. All shRNAs were cloned into pLKO.1 hygro (a gift from Bob Weinberg; Addgene plasmid #24150, RRID:Addgene_24150). shRNA knockdown and GLuc-T2A-EGFP-expressing lines were generated through lentiviral transduction. .. Lentiviral particles were produced by co-transfecting 293T cells with pLKO.1 hygro lentiviral constructs or pLV GLuc-T2A-EGFP (VectorBuilder), along with pMDLg/pRRE, pRSV-Rev, and pMD2.g (gifts from Didier Trono.

    Transduction:

    Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function
    Article Snippet: .. All shRNAs were cloned into pLKO.1 hygro (a gift from Bob Weinberg; Addgene plasmid #24150, RRID:Addgene_24150). shRNA knockdown and GLuc-T2A-EGFP-expressing lines were generated through lentiviral transduction. .. Lentiviral particles were produced by co-transfecting 293T cells with pLKO.1 hygro lentiviral constructs or pLV GLuc-T2A-EGFP (VectorBuilder), along with pMDLg/pRRE, pRSV-Rev, and pMD2.g (gifts from Didier Trono.


    Mass Spectrometry:

    Article Title: Regulation of mitophagy by Fis1 and Fascin1-organized actin.
    Article Snippet: Article

    Microscopy:

    Article Title: Regulation of mitophagy by Fis1 and Fascin1-organized actin.
    Article Snippet: Article

    Flow Cytometry:

    Article Title: Regulation of mitophagy by Fis1 and Fascin1-organized actin.
    Article Snippet: Article

    Sequencing:

    Article Title: Regulation of mitophagy by Fis1 and Fascin1-organized actin.
    Article Snippet: Article

    Article Title: Interaction and Reciprocal Stabilization of H3.3 and PARP1 Promotes DNA Damage Repair and Tumor Progression in Small Cell Lung Cancer
    Article Snippet: 28 Multiple lines of evidence have highlighted the transcriptional regulatory activity of H3.3 at promoters and 29 telomeres.. However, H3.3 may also play transcriptional regulation-independent roles.. In this study, we 30 demonstrated that overexpression of H3F3A, the gene encoding H3.3, supported the pathogenesis of 31 small cell lung cancer (SCLC) by protecting PARP1 from degradation.

    Expressing:

    Article Title: Regulation of mitophagy by Fis1 and Fascin1-organized actin.
    Article Snippet: Article


    Negative Control:

    Article Title: Enhanced therapeutic efficacy of anti-PD-1 blockade by targeting LAMP2A inhibits lysosomal degradation of STING and TBK1
    Article Snippet: The chemicals used were listed as follows: NH4Cl (Ammonium chloride, A9434, Sigma, St. Louis, MO, USA), Leupeptin (103476-89-7, MP Biomedicals, California, USA), MG132 (S2619, Selleck), and CHX (cycloheximide, S7418, Selleck), AKTi-1/2(AKT inhibitor VIII, HY-10355, MCE), AR7 (HY-101106, MCE), Rapamycin(553210, Sigma), D-Luciferin Sodium Salt (40901ES, Yeasen, China), C-176(SML2559, Sigma), GSK8612(SML2721, Sigma), Diclofenac Sodium (D6899, Sigma). .. Single guide RNAs (sgRNAs) were cloned into lentiCRISPR v2 (Addgene # 52961) and shRNA were cloned into pLKO.1 puro (Addgene #8453) or pLKO.1 hygro(Addgene #24150). siRNAs targeting siLAMP2A, siATG5, siATG7 and negative control (NC) siRNA transfected into cells with a riboFECTTM CP Transfection Kit (RiboBio). ..

    Transfection:

    Article Title: Enhanced therapeutic efficacy of anti-PD-1 blockade by targeting LAMP2A inhibits lysosomal degradation of STING and TBK1
    Article Snippet: The chemicals used were listed as follows: NH4Cl (Ammonium chloride, A9434, Sigma, St. Louis, MO, USA), Leupeptin (103476-89-7, MP Biomedicals, California, USA), MG132 (S2619, Selleck), and CHX (cycloheximide, S7418, Selleck), AKTi-1/2(AKT inhibitor VIII, HY-10355, MCE), AR7 (HY-101106, MCE), Rapamycin(553210, Sigma), D-Luciferin Sodium Salt (40901ES, Yeasen, China), C-176(SML2559, Sigma), GSK8612(SML2721, Sigma), Diclofenac Sodium (D6899, Sigma). .. Single guide RNAs (sgRNAs) were cloned into lentiCRISPR v2 (Addgene # 52961) and shRNA were cloned into pLKO.1 puro (Addgene #8453) or pLKO.1 hygro(Addgene #24150). siRNAs targeting siLAMP2A, siATG5, siATG7 and negative control (NC) siRNA transfected into cells with a riboFECTTM CP Transfection Kit (RiboBio). ..



    Similar Products

    94
    Addgene inc addgene plasmid
    Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+hygro/pLKO%2E1+hygro+(Plasmid+%2324150)/pm41915470-258-17-17
    Average 94 stars, based on 1 article reviews
    addgene plasmid - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc plko 1 hygro stewart
    Plko 1 Hygro Stewart, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+hygro/pLKO%2E1+hygro+(Plasmid+%2324150)/pm41915470-258-12-17
    Average 94 stars, based on 1 article reviews
    plko 1 hygro stewart - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc plko 1 hygro
    Plko 1 Hygro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+hygro/pLKO%2E1+hygro+(Plasmid+%2324150)/pm41742405-330-132-135
    Average 94 stars, based on 1 article reviews
    plko 1 hygro - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc paper n a plko 1 hygro addgene
    Paper N A Plko 1 Hygro Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+hygro/pLKO%2E1+hygro+(Plasmid+%2324150)/pm41742405-307-173-177
    Average 94 stars, based on 1 article reviews
    paper n a plko 1 hygro addgene - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc plko 1
    Plko 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+hygro/pLKO%2E1+hygro+(Plasmid+%2324150)/pmc13004551-112-0-2
    Average 94 stars, based on 1 article reviews
    plko 1 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc addgene 24150
    Addgene 24150, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+hygro/pLKO%2E1+hygro+(Plasmid+%2324150)/pm41722050-802-134-134
    Average 94 stars, based on 1 article reviews
    addgene 24150 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc plko 1 plasmid
    Plko 1 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+hygro/pLKO%2E1+hygro+(Plasmid+%2324150)/pm41381491-305-20-23
    Average 94 stars, based on 1 article reviews
    plko 1 plasmid - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Addgene inc plko
    Plko, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+hygro/pLKO%2E1+hygro+(Plasmid+%2324150)/pm41310344-417-26-29
    Average 94 stars, based on 1 article reviews
    plko - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results